Day :
Keynote Forum
Irene Cantone
Doctor , University of Naples Federico
Keynote: Understanding epigenetic reprogramming by X chromosome reactivation
Time : 13:00-13:30
Biography:
Irene Cantone has completed his PhD at the age of 25 years from Telethon Institute of Genetic and Medicine and postdoctoral studies from MRC London institute of Medical Sciences. She has been awarded Marie-Curie, EMBO and Human Frontiers Science Programme long-term fellowship. She is currently a Professor at the University. She has published more than 15 papers in reputed peerreviewed journals (e.g. Cell, Nature Structural Molecular Biology, Nature Communication and Genome Biology) and has been serving as an editorial board member of PLOS and Nature journals.
Abstract:
rasure of epigenetic memory is required to convert somatic cells towards pluripotency. Reactivation of the inactive X chromosome (Xi) has been used to model epigenetic reprogramming in mouse, but human studies are hampered by Xi epigenetic instability and difficulties in tracking partially reprogrammed iPSCs. Recently, I have established a cell fusion reprogramming system that recapitulates features of human naïve pluripotency and enables tracing early chromatin changes. This system revealed that loss of XIST and H3K27me3 from the human Xi precedes and is required for Xi transcriptional reactivation ahead of cell division (Cantone et al., Nature Comm 2016). Interestingly, single-cell RNA-FISH and allele-specific RNA sequencing analyses revealed that reprogramming-mediated human Xi reactivation was partial and selective for a specific subset of genes. Selective Xi reactivation was not limited to gene loci residing within specific chromatin domains (e.g. H3K27me3 or H3K9me3 domains) neither influenced by proximity to XIST locus. Reactivation was instead associated with stochastic Xi expression ahead of reprogramming, as shown by single cells and isogenic fibroblast clones (Cantone et al., Genome Biology 2017). Notably, stochastic Xi transcription is stabilized in some clonal lineages suggesting that single-cell transcriptional variability might underlie heritable gene reactivation even in heterochromatic contexts. Implications for targeted Xi gene reactivation during human pluripotent reprogramming and in somatic cells will be discussed as a concept for modelling and therapy of human X-linked diseases (
Keynote Forum
Samir Rafla
Alexandria University, Egypt
Keynote: ECG in athletes, limits of norma
Time : 13:00-13:30
Biography:
Samir Morcos Rafla Emeritus professor of cardiology, Alexandria University, Egypt Fellow American College of Cardiology FACC Emeritus Fellow European Society of Cardiology EFESC Fellow Heart Rhythm Society FHRS (American society of arrhythmia) Member European Heart Rhythm Society HER Reviewer in many journals and assessor of abstracts and papers in conferences. Publications in Researchgate and Google Scholar.
Abstract:
Sudden cardiac arrest in apparently normal hearts. Ablation of atrial fibrillation, radiofrequency versus cryoablation. Value of treating COPD associated with heart failure, the impact of therapy on both conditions. QRS duration after myocardial infarction, the impact of thrombolytic therapy.
Keynote Forum
Roshinipriya Ganesin
Selayang Hospital, Malaysia
Keynote: econdary prophylaxis for rheumatic heart disease: A case report
Time : 13:00-13:30
Biography:
Roshinipriya Ganesin has completed her education in department of medicine from International Medicalo University Clinical campus Kluang, Malaysia. Currently she is working in Selayang Hospital, Malaysia.
Abstract:
Rheumatic heart disease is an irreversible sequela of rheumatic fever which is a major cause of cardiovascular morbidity and mortality in developing countries. We report a case of a 22-year-old lady with underlying chronic rheumatic heart disease, underwent mitral valve repair, presented with a recurrence of acute rheumatic fever with new aortic regurgitation confirmed by echocardiography, upon defaulting oral penicillin prophylaxis. She was treated symptomatically and started on IV Penicillin 2.4MU for 14 days based on clinical suspicion of possible infective endocarditis although laboratory values were not suggestive. With the resolution of clinical symptoms and treatment completion, she was discharged with oral penicillin despite non-compliance and a referral to the tertiary cardiac centre for continuation of care. Medical management was continued without surgical intervention to date. There is no specific guideline available in Malaysia to manage rheumatic heart disease, therefore treatment relies on the physician’s experience. Majority still prefer prescribing oral penicillin prophylaxis in Malaysia but the recommended choice of prophylaxis worldwide is IM penicillin. In my opinion, this patient should have been discharged with IM penicillin or, even better, initiated at the point of initial diagnosis. In this case report, we not only highlight the importance of secondary prophylaxis but also focus on the route of prophylaxis to halt the progression of the disease.